On matchday 1, both teams ended in an exciting 2-2 draw at Stamford Bridge but this time round the Italian champions and the title holders eye a place in the knockout stages of the competition.
Though Chelsea are joint-top of the Group, only a point separates them from Tuesday nights opponents and a win for either side could increase their chances of qualification depending on the other result.
Juve were held 0-0 at the weekend against Lazio but are still on top of Serie A table while Roberto Di Matteo's side have lost twice in four games, dropping to third place in the title race.
Angelo Alessio's side have no fresh injury worries as playmaker Andrea Pirlo returns to the side after serving a domestic ban but striker Mirko Vucinic is still sidelined.
Alessandro Matri and Nicklas Bendtner will be fighting for a place in the squad as Sebastian Giovinco's poor show against Lazio.
On the other hand, Di Matteo has admitted that Daniel Sturridge could be included in the starting XI in place of Fernando Torres after the Spaniard's lacklustre performance in 2-1 defeat to West Brom Albion.
Oscar, Juan Mata, Ramirez, Ashley Cole and Branislav Ivanovic started on the bench in that loss, but are expected to start against Juventus on Tuesday.
Meanwhile, Chelsea miss their influencial skipper John Terry through knee injury while midfielder Frank Lampard is still sidelined.
*Juventus have scored 197 goals in the UEFA Champions League.
*Chelsea have scored 197 goals in the European cup and conceded 145 in UEFA competition.
Juventus (3-5-2): Buffon-Barzagli, Bonucci, Chiellini-Lichtsteiner, Marchisio, Pirlo, Vidal, Isla-Quagliarella, Giovinco.
Chelsea (4-2-3-1): Cech-Ivanovic, Cahill, Luiz, Cole-Mikel, Ramires-Mata, Oscar, Hazard-Torres.