The accused -- Obaid-ur-Rehman, Hilal-ud-din and Nafikul -- appeared before Additional District and Sessions Judge (III) Rajendra Chandra under tight security, where the cross-questioning of Dr R N Singh was completed.
There are 48 witnesses in the case, of which 26 have recorded their statements so far.
Setting tomorrow as date of the next hearing of the case, the court ordered a non-bailable warrant of Rs 2,000 for 27th witness Dr R A Chakravarthi for not appearing before it today.
The court also summoned 28th witness Dr N Singh after he did not appear before it either.
Charges against the accused have already been filed under sections 148, 307, 302 and 120 B of the CrPC, section 3/4 of the Explosive Substance Act, section 150 and 151 of the Railway Act and section 14 of the Foreigners Act.
Two other accused -- Mohd Sharif, alias Sirajuddin, of Bangladesh, and Mohd Roni -- are absconding.
Another accused, Ghulam Rajdani, alias Pahiya, was gunned down in an encounter.
In May last, Hilal-ud-din and Nafikul were brought here from Cherallapalli Central Jail, Andhra Pradesh, on a warrant, while Obaid-ur-Rehman, a Bangladeshi, was brought from Murshidabad, West Bengal.
The July 28, 2005 blast near Halpalganj-Singramau railway station in the district had killed at least 14 people and severely wounded 90 others.